Firefly Luciferase circRNA is a circular mRNA molecule encoding a luciferase protein, and the expressed protein was originally isolated from fireflies. Firefly luciferase is a commonly used bioluminescence reporter molecule in studies on gene regulation, cell viability assays, ATP level analysis, in vivo imaging, etc. The luminescence of luciferase differs from fluorescence in that no excitation light is required, but requires a substrate. In the presence of ATP and oxygen, luciferase catalyzes the oxidation of substrates to fluoresce at 560 nm.

This product is the high-purity circular mRNA molecules produced after circularization and purification, and is resistant to RNaseR digestion. The circular mRNA product has stronger stability than linear mRNA, and presents more durable protein expression than linear mRNA in cell experiments and animal experiments.

The followings are the Diagram of Capillary Electrophoresis (CE) Purity Identification for Prepared Firefly Luciferase circRNA, and the Expression Diagram in Mice.

CE Identification Diagram

Living Imaging of Expression in Mice